Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2.

ABSTRACT: Heterochromatin protein 1 (HP1) family proteins are conserved chromatin binding proteins involved in gene silencing, chromosome packaging, and chromosome segregation. These proteins recognize histone H3 lysine 9 methylated tails via their chromodomain and recruit additional ligand proteins with diverse activities through their dimerization domain, the chromoshadow domain. Species that have HP1 proteins possess multiple paralogs that perform non-overlapping roles in vivo. How different HP1 proteins, which are highly conserved, perform different functions is not well understood. Here, we use the two Schizosaccharomyces pombe HP1 paralogs, Swi6 and Chp2, as model systems to compare and contrast their biophysical properties. We find that Swi6 and Chp2 have similar dimerization and oligomerizat

SUBMITTER: Isaac RS 

PROVIDER: S-EPMC5693750 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature