Cryogenic Vibrational Spectroscopy Provides Unique Fingerprints for Glycan Identification.

ABSTRACT: The structural characterization of glycans by mass spectrometry is particularly challenging. This is because of the high degree of isomerism in which glycans of the same mass can differ in their stereochemistry, attachment points, and degree of branching. Here we show that the addition of cryogenic vibrational spectroscopy to mass and mobility measurements allows one to uniquely identify and characterize these complex biopolymers. We investigate six disaccharide isomers that differ in their stereochemistry, attachment point of the glycosidic bond, and monosaccharide content, and demonstrate that we can identify each one unambiguously. Even disaccharides that differ by a single stereogenic center or in the monosaccharide sequence order show distinct vibrational fingerprints that would clear

SUBMITTER: Masellis C 

PROVIDER: S-EPMC5693781 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature