Exenatide Inhibits the K_Ca3.1 Channels of Aortic Vascular Smooth Muscle in Diabetic Rats.

ABSTRACT:

Background

K_Ca3.1 ion channels play an important role during atherosclerosis. We aimed to investigate the effect of exenatide on K_Ca3.1 expression in aortic vascular smooth muscle cells (VSMCs) of diabetic rats.

Methods

Sprague-Dawley rats were randomly divided into normal control (NC), diabetes model (DM), and exenatide treatment (ET) groups. Hematoxylin and eosin and α-actin immunohistochemical staining were used to detect changes in rat aortic vascular smooth muscle. Quantitative RT-PCR and Western blot analysis were used to detect changes in K_Ca3.1 mRNA and protein levels, respectively.

Results

Aortic tissue staining in the DM group revealed an absence of smooth or integrated endothelium, increased smooth muscle cell proliferation

SUBMITTER: Dong P 

PROVIDER: S-EPMC5694930 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature