Translational control of lipogenic enzymes in the cell cycle of synchronous, growing yeast cells.

ABSTRACT: Translational control during cell division determines when cells start a new cell cycle, how fast they complete it, the number of successive divisions, and how cells coordinate proliferation with available nutrients. The translational efficiencies of mRNAs in cells progressing synchronously through the mitotic cell cycle, while preserving the coupling of cell division with cell growth, remain uninvestigated. We now report comprehensive ribosome profiling of a yeast cell size series from the time of cell birth, to identify mRNAs under periodic translational control. The data reveal coordinate translational activation of mRNAs encoding lipogenic enzymes late in the cell cycle including Acc1p, the rate-limiting enzyme acetyl-CoA carboxylase. An upstream open reading frame (uORF) confers the t

SUBMITTER: Blank HM 

PROVIDER: S-EPMC5694946 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature