Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens.

ABSTRACT: Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 10⁶ and 10⁸ copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg

SUBMITTER: Safavieh M 

PROVIDER: S-EPMC5695240 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature