Mass spectrometry-based quantification of the cellular response to ultraviolet radiation in HeLa cells.

ABSTRACT: Ultraviolet (UV) irradiation is a common form of DNA damage that can cause pyrimidine dimers between DNA, which can cause gene mutations, even double-strand breaks and threaten genome stability. If DNA repair systems default their roles at this stage, the organism can be damaged and result in disease, especially cancer. To better understand the cellular response to this form of damage, we applied highly sensitive mass spectrometry to perform comparative proteomics of phosphorylation in HeLa cells. A total of 4367 phosphorylation sites in 2100 proteins were identified, many of which had not been reported previously. Comprehensive bioinformatics analysis revealed that these proteins were involved in many important biological processes, including signaling, localization and cell cycle regulat

SUBMITTER: Xu H 

PROVIDER: S-EPMC5695813 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature