Label-free fiber optic optrode for the detection of class C β-lactamases expressed by drug resistant bacteria.

ABSTRACT: This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C β-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to β-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid-based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration. In order to prove the feasibility analysis towards a fully automated optical assay, an engineered system was developed to simultaneously manipulate and interrogate multiple fiber optic optrodes in the different phases of

SUBMITTER: Zuppolini S 

PROVIDER: S-EPMC5695963 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature