Direct comparison of derivatization strategies for LC-MS/MS analysis of N-glycans.

ABSTRACT: Protein glycosylation is a common post-translational modification that has significant impacts on protein folding, lifespan, conformation, distribution and function. N-Glycans, which are attached to asparagine residues of proteins, are studied most often due to their compatibility with enzymatic release. Despite the ease of N-glycan release, compositional and structural complexity coupled with poor ionization efficiency during liquid chromatography mass spectrometry (LC-MS) make quantitative glycomic studies a significant challenge. To overcome these challenges, glycans are almost always derivatized prior to LC-MS analyses to impart favorable characteristics, such as improved ionization efficiency, increased LC separation efficiency and the production of more informative fragments during t

SUBMITTER: Zhou S 

PROVIDER: S-EPMC5696090 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature