Removal of ligand-bound liposomes from cell surfaces by microbubbles exposed to ultrasound.

ABSTRACT: Gas-filled microbubbles attached to cell surfaces can interact with focused ultrasound to create microstreaming of nearby fluid. We directly observed the ultrasound/microbubble interaction and documented that under certain conditions fluorescent particles that were attached to the surface of live cells could be removed. Fluorescently labeled liposomes that were larger than 500 nm in diameter were attached to the surface of endothelial cells using cRGD targeting to αvβ3 integrin. Microbubbles were attached to the surface of the cells through electrostatic interactions. Images taken before and after the ultrasound exposure were compared to document the effects on the liposomes. When exposed to ultrasound with peak negative pressure of 0.8 MPa, single microbubbles and groups of isolated micro

SUBMITTER: Ibsen S 

PROVIDER: S-EPMC5696303 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature