Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair.

ABSTRACT: Efficient cell membrane repair mechanisms are essential for maintaining membrane integrity and thus for cell life. Here we show that the Ca²⁺- and phospholipid-binding proteins annexin A4 and A6 are involved in plasma membrane repair and needed for rapid closure of micron-size holes. We demonstrate that annexin A4 binds to artificial membranes and generates curvature force initiated from free edges, whereas annexin A6 induces constriction force. In cells, plasma membrane injury and Ca²⁺ influx recruit annexin A4 to the vicinity of membrane wound edges where its homo-trimerization leads to membrane curvature near the edges. We propose that curvature force is utilized together with annexin A6-mediated constriction force to pull the wound edges together for eventual fusi

SUBMITTER: Boye TL 

PROVIDER: S-EPMC5696365 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature