Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling.

ABSTRACT: The mammary epithelium comprises two primary cellular lineages, but the degree of heterogeneity within these compartments and their lineage relationships during development remain an open question. Here we report single-cell RNA profiling of mouse mammary epithelial cells spanning four developmental stages in the post-natal gland. Notably, the epithelium undergoes a large-scale shift in gene expression from a relatively homogeneous basal-like program in pre-puberty to distinct lineage-restricted programs in puberty. Interrogation of single-cell transcriptomes reveals different levels of diversity within the luminal and basal compartments, and identifies an early progenitor subset marked by CD55. Moreover, we uncover a luminal transit population and a rare mixed-lineage cluster amongst basa

SUBMITTER: Pal B 

PROVIDER: S-EPMC5696379 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature