A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage.

ABSTRACT: The bacterial SOS stress-response pathway is a pro-mutagenic DNA repair system that mediates bacterial survival and adaptation to genotoxic stressors, including antibiotics and UV light. The SOS pathway is composed of a network of genes under the control of the transcriptional repressor, LexA. Activation of the pathway involves linked but distinct events: an initial DNA damage event leads to activation of RecA, which promotes autoproteolysis of LexA, abrogating its repressor function and leading to induction of the SOS gene network. These linked events can each independently contribute to DNA repair and mutagenesis, making it difficult to separate the contributions of the different events to observed phenotypes. We therefore devised a novel synthetic circuit to unlink these events and perm

SUBMITTER: Kubiak JM 

PROVIDER: S-EPMC5696648 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature