Sequence and Solution Effects on the Prevalence of d-Isomers Produced by Deamidation.

ABSTRACT: Deamidation of asparagine is a spontaneous and irreversible post-translational modification associated with a growing list of human diseases. While pervasive, deamidation is often overlooked because it represents a relatively minor chemical change. Structural and functional characterization of this modification is complicated because deamidation of asparagine yields four isomeric forms of Asp. Herein, radical directed dissociation (RDD), in conjunction with mass spectrometry, is used to identify and quantify all four isomers in a series of model peptides that were subjected to various deamidation conditions. Although primary sequence significantly influences the rate of deamidation, it has little impact on the relative proportions of the product isomers. Furthermore, the addition of ammoni

SUBMITTER: Riggs DL 

PROVIDER: S-EPMC5696650 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature