Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane.

ABSTRACT:

Background

In thylakoid membrane, each monomer of the dimeric complex of cytochrome b ₆ f is comprised of eight subunits that are both nucleus- and plastid-encoded. Proper cytochrome b ₆ f complex integration into the thylakoid membrane requires numerous regulatory factors for coordinated transport, insertion and assembly of the subunits. Although, the chloroplast-encoded cytochrome b ₆ f subunit IV (PetD) consists of three transmembrane helices, the signal and the mechanism of protein integration into the thylakoid membrane have not been identified.

Results

Here, we demonstrate that the native PetD subunit cannot incorporate into the thylakoid membranes spontaneously, but that proper integration occurs through the post-translational signal r

SUBMITTER: Kroliczewski J 

PROVIDER: S-EPMC5697057 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature