A validated antibody panel for the characterization of tau post-translational modifications.

ABSTRACT:

Background

Tau is a microtubule-binding protein, which is subject to various post-translational modifications (PTMs) including phosphorylation, methylation, acetylation, glycosylation, nitration, sumoylation and truncation. Aberrant PTMs such as hyperphosphorylation result in tau aggregation and the formation of neurofibrillary tangles, which are a hallmark of Alzheimer's disease (AD). In order to study the importance of PTMs on tau function, antibodies raised against specific modification sites are widely used. However, quality control of these antibodies is lacking and their specificity for particular modifications is often unclear.

Methods

In this study, we first designed an online tool called 'TauPTM', which enables the visualization of PTMs and their interactions on hum

SUBMITTER: Ercan E 

PROVIDER: S-EPMC5697095 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature