Nucleic acid purification from plants, animals and microbes in under 30 seconds.

ABSTRACT: Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed. Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from diffi

SUBMITTER: Zou Y 

PROVIDER: S-EPMC5697807 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature