Listeria monocytogenes Replicate in Bone Marrow-Derived CD11c⁺ Cells but Not in Dendritic Cells Isolated from the Murine Gastrointestinal Tract.

ABSTRACT: Recent fate-mapping studies and gene-expression profiles suggest that commonly used protocols to generate bone marrow-derived cultured dendritic cells yield a heterogeneous mixture, including some CD11c^hi cells that may not have a bona fide counterpart in vivo. In this study, we provide further evidence of the discordance between ex vivo-isolated and in vitro-cultured CD11c⁺ cells by analyzing an additional phenotype, the ability to support cytosolic growth of the facultative intracellular bacterial pathogen Listeria monocytogenes Two days after foodborne infection of mice with GFP-expressing L. monocytogenes, a small percentage of CD103^neg and CD103⁺ conventional dendritic cells (cDC) in the intestinal lamina propria and mesenteric

SUBMITTER: Jones GS 

PROVIDER: S-EPMC5698106 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature