Accurate quantification of PGE₂ in the polyposis in rat colon (Pirc) model by surrogate analyte-based UPLC-MS/MS.

ABSTRACT: An accurate and reliable UPLC-MS/MS method is reported for the quantification of endogenous Prostaglandin E2 (PGE₂) in rat colonic mucosa and polyps. This method adopted the "surrogate analyte plus authentic bio-matrix" approach, using two different stable isotopic labeled analogs - PGE₂-d9 as the surrogate analyte and PGE₂-d4 as the internal standard. A quantitative standard curve was constructed with the surrogate analyte in colonic mucosa homogenate, and the method was successfully validated with the authentic bio-matrix. Concentrations of endogenous PGE₂ in both normal and inflammatory tissue homogenates were back-calculated based on the regression equation. Because of no endogenous interference on the surrogate analyte determination, the spe

SUBMITTER: Yun C 

PROVIDER: S-EPMC5698167 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature