Functional significance of C-terminal mobile domain of cardiac troponin I.

ABSTRACT: Ca²⁺-regulation of cardiac contractility is mediated through the troponin complex, which comprises three subunits: cTnC, cTnI, and cTnT. As intracellular [Ca²⁺] increases, cTnI reduces its binding interactions with actin to primarily interact with cTnC, thereby enabling contraction. A portion of this regulatory switching involves the mobile domain of cTnI (cTnI-MD), the role of which in muscle contractility is still elusive. To study the functional significance of cTnI-MD, we engineered two cTnI constructs in which the MD was truncated to various extents: cTnI(1-167) and cTnI(1-193). These truncations were exchanged for endogenous cTnI in skinned rat papillary muscle fibers, and their influence on Ca²⁺-activated contraction and cross-bridge cycling kinetics

SUBMITTER: Bohlooli Ghashghaee N 

PROVIDER: S-EPMC5698173 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature