An organic fluorophore-nanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing.

ABSTRACT: Organic fluorescent probes are widely used to detect key biomolecules; however, they often lack the photostability required for extended intracellular imaging. Here we report a new hybrid nanomaterial (peroxynanosensor, PNS), consisting of an organic fluorescent probe bound to a nanodiamond, that overcomes this limitation to allow concurrent and extended cell-based imaging of the nanodiamond and ratiometric detection of hydrogen peroxide. Far-red fluorescence of the nanodiamond offers continuous monitoring without photobleaching, while the green fluorescence of the organic fluorescent probe attached to the nanodiamond surface detects hydrogen peroxide on demand. PNS detects basal production of hydrogen peroxide within M1 polarised macrophages and does not affect macrophage growth during pr

SUBMITTER: Purdey MS 

PROVIDER: S-EPMC5698319 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature