HDL particles incorporate into lipid bilayers - a combined AFM and single molecule fluorescence microscopy study.

ABSTRACT: The process, how lipids are removed from the circulation and transferred from high density lipoprotein (HDL) - a main carrier of cholesterol in the blood stream - to cells, is highly complex. HDL particles are captured from the blood stream by the scavenger receptor, class B, type I (SR-BI), the so-called HDL receptor. The details in subsequent lipid-transfer process, however, have not yet been completely understood. The transfer has been proposed to occur directly at the cell surface across an unstirred water layer, via a hydrophobic channel in the receptor, or after HDL endocytosis. The role of the target lipid membrane for the transfer process, however, has largely been overlooked. Here, we studied at the single molecule level how HDL particles interact with synthetic lipid membranes. U

SUBMITTER: Plochberger B 

PROVIDER: S-EPMC5698431 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature