AFM-based detection of glycocalyx degradation and endothelial stiffening in the db/db mouse model of diabetes.

ABSTRACT: Degradation of the glycocalyx and stiffening of endothelium are important pathophysiological components of endothelial dysfunction. However, to our knowledge, these events have not been investigated in tandem in experimental diabetes. Here, the mechanical properties of the glycocalyx and endothelium in ex vivo mouse aorta were determined simultaneously in indentation experiments with an atomic force microscope (AFM) for diabetic db/db and control db/+ mice at ages of 11-19 weeks. To analyze highly heterogeneous aorta samples, we developed a tailored classification procedure of indentation data based on a bi-layer brush model supplemented with Hertz model for quantification of nanomechanics of endothelial regions with and without the glycocalyx surface. In db/db mice, marked endothelial sti

SUBMITTER: Targosz-Korecka M 

PROVIDER: S-EPMC5698475 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature