A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.

ABSTRACT: Binary expression systems have revolutionised genetic research by enabling delivery of loss-of-function and gain-of-function transgenes with precise spatial-temporal resolution in vivo. However, at present, each existing platform relies on a defined exogenous transcription activator capable of binding a unique recognition sequence. Consequently, none of these technologies alone can be used to simultaneously target different tissues or cell types in the same organism. Here, we report a modular system based on programmable transcription activator-like effector (TALE) proteins, which enables parallel expression of multiple transgenes in spatially distinct tissues in vivo. Using endogenous enhancers coupled to TALE drivers, we demonstrate multiplexed orthogonal activation of several transgenes

SUBMITTER: Toegel M 

PROVIDER: S-EPMC5698491 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature