High-throughput characterization of protein-protein interactions by reprogramming yeast mating.

ABSTRACT: High-throughput methods for screening protein-protein interactions enable the rapid characterization of engineered binding proteins and interaction networks. While existing approaches are powerful, none allow quantitative library-on-library characterization of protein interactions in a modifiable extracellular environment. Here, we show that sexual agglutination of Saccharomyces cerevisiae can be reprogrammed to link interaction strength with mating efficiency using synthetic agglutination (SynAg). Validation of SynAg with 89 previously characterized interactions shows a log-linear relationship between mating efficiency and protein binding strength for interactions with K_ds ranging from below 500 pM to above 300 μM. Using induced chromosomal translocation to pair b

SUBMITTER: Younger D 

PROVIDER: S-EPMC5699035 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature