O₂ availability impacts iron homeostasis in Escherichia coli.

ABSTRACT: The ferric-uptake regulator (Fur) is an Fe²⁺-responsive transcription factor that coordinates iron homeostasis in many bacteria. Recently, we reported that expression of the Escherichia coli Fur regulon is also impacted by O₂ tension. Here, we show that for most of the Fur regulon, Fur binding and transcriptional repression increase under anaerobic conditions, suggesting that Fur is controlled by O₂ availability. We found that the intracellular, labile Fe²⁺ pool was higher under anaerobic conditions compared with aerobic conditions, suggesting that higher Fe²⁺ availability drove the formation of more Fe²⁺-Fur and, accordingly, more DNA binding. O₂ regulation of Fur activity required the anaerobically induce

SUBMITTER: Beauchene NA 

PROVIDER: S-EPMC5699043 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature