PH and Potential Transients of the bc₁ Complex Co-Reconstituted in Proteo-Lipobeads with the Reaction Center from Rb. sphaeroides.

ABSTRACT: His-tag technology is employed to bind membrane proteins, such as the bc₁ complex and the reaction center (RC) from Rhodobacter sphaeroides, to spherical as well as planar surfaces in a strict orientation. Subsequently, the spherical and planar surfaces are subjected to in situ dialysis to form proteo-lipobeads (PLBs) and protein-tethered bilayer membranes, respectively. PLBs based on Ni-nitrileotriacetic acid-functionalized agarose beads that have diameters ranging from 50 to 150 μm are used to assess proton release and membrane potential parameters by confocal laser-scanning microscopy. The pH and potential transients are thus obtained from bc₁ activated by the RC. To assess the turnover of bc₁ excited by the RC in a similar setting, we used the planar su

SUBMITTER: Geiss AF 

PROVIDER: S-EPMC5699462 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature