Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440.

ABSTRACT: Pseudomonas putida strains are highly robust bacteria known for their ability to efficiently utilize a variety of carbon sources, including aliphatic and aromatic hydrocarbons. Recently, P. putida has been engineered to valorize the lignin stream of a lignocellulosic biomass pretreatment process. Nonetheless, when compared to platform organisms such as Escherichia coli, the toolkit for engineering P. putida is underdeveloped. Heterologous gene expression in particular is problematic. Plasmid instability and copy number variance provide challenges for replicative plasmids, while use of homologous recombination for insertion of DNA into the chromosome is slow and laborious. Further, most heterologous expression efforts to date typically rely on overexpression of e

SUBMITTER: Elmore JR 

PROVIDER: S-EPMC5699527 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature