A comprehensive evaluation of constraining amino acid biosynthesis in compartmented models for metabolic flux analysis.

ABSTRACT: Recent advances in the availability and applicability of genetic tools for non-conventional yeasts have raised high hopes regarding the industrial applications of such yeasts; however, quantitative physiological data on these yeasts, including intracellular flux distributions, are scarce and have rarely aided in the development of novel yeast applications. The compartmentation of eukaryotic cells adds to model complexity. Model constraints are ideally based on biochemical evidence, which is rarely available for non-conventional yeast and eukaryotic cells. A small-scale model for 13C-based metabolic flux analysis of central yeast carbon metabolism was developed that is universally valid and does not depend on localization information regarding amino acid anabolism. The variable compartmenta

SUBMITTER: Lehnen M 

PROVIDER: S-EPMC5699530 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature