Mast Cell-Derived Tryptase in Geographic Atrophy.

ABSTRACT:

Purpose

Our previous study demonstrated significantly more degranulating mast cells (MCs) in choroids from subjects with age-related macular degeneration compared to aged controls. This study examined the immunolocalization of tryptase, the most abundant MC secretory granule-derived serine protease, in aged control eyes and eyes with geographic atrophy (GA).

Methods

Postmortem human eyes with and without GA were obtained from the National Disease Research Interchange. Tissue was fixed, cryopreserved, sectioned, and immunostained with a monoclonal antibody against tryptase. Sections were imaged on a Zeiss 710 Confocal Microscope.

Results

In the posterior pole of all aged control eyes, tryptase was confined to choroidal MCs, which were located primarily in Sattler's lay

SUBMITTER: McLeod DS 

PROVIDER: S-EPMC5699534 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature