Insulin-dependent metabolic and inotropic responses in the heart are modulated by hydrogen peroxide from NADPH-oxidase isoforms NOX2 and NOX4.

ABSTRACT:

Rationale

Hydrogen peroxide (H₂O₂) is a stable reactive oxygen species (ROS) that has long been implicated in insulin signal transduction in adipocytes. However, H₂O₂'s role in mediating insulin's effects on the heart are unknown.

Objective

We investigated the role of H₂O₂ in activating insulin-dependent changes in cardiac myocyte metabolic and inotropic pathways. The sources of insulin-dependent H₂O₂ generation were also studied.

Methods and results

In addition to the canonical role of insulin in modulating cardiac metabolic pathways, we found that insulin also inhibited beta adrenergic-induced increases in cardiac contractility. Catalase and NADPH oxidase (NOX) inhibitors blunted

SUBMITTER: Steinhorn B 

PROVIDER: S-EPMC5699944 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature