Limitations of oxygen delivery to cells in culture: An underappreciated problem in basic and translational research.

ABSTRACT: Molecular oxygen is one of the most important variables in modern cell culture systems. Fluctuations in its concentration can affect cell growth, differentiation, signaling, and free radical production. In order to maintain culture viability, experimental validity, and reproducibility, it is imperative that oxygen levels be consistently maintained within physiological "normoxic" limits. Use of the term normoxia, however, is not consistent among scientists who experiment in cell culture. It is typically used to describe the atmospheric conditions of a standard incubator, not the true microenvironment to which the cells are exposed. This error may lead to the situation where cells grown in a standard "normoxic" oxygen concentration may actually be experiencing a wide range of conditions rang

SUBMITTER: Place TL 

PROVIDER: S-EPMC5699948 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature