Down-regulation of NOX2 activity in phagocytes mediated by ATM-kinase dependent phosphorylation.

ABSTRACT: NADPH oxidases (NOX) have many biological roles, but their regulation to control production of potentially toxic ROS molecules remains unclear. A previously identified insertion sequence of 21 residues (called NIS) influences NOX activity, and its predicted flexibility makes it a good candidate for providing a dynamic switch controlling the NOX active site. We constructed NOX2 chimeras in which NIS had been deleted or exchanged with those from other NOXs (NIS1, 3 and 4). All contained functional heme and were expressed normally at the plasma membrane of differentiated PLB-985 cells. However, NOX2-ΔNIS and NOX2-NIS1 had neither NADPH-oxidase nor reductase activity and exhibited abnormal translocation of p47^phox and p67^phox to the phagosomal membrane. This suggested a f

SUBMITTER: Beaumel S 

PROVIDER: S-EPMC5699957 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature