Isolation and functional identification of three cuticle protein genes during metamorphosis of the beet armyworm, Spodoptera exigua.

ABSTRACT: The beet armyworm, Spodoptera exigua (Hubner), is one of the major crop pests and is a target for current pest control approaches using insecticides. In this study three cuticular protein genes CPG316, CPG860 and CPG4855 have been cloned from 0 h pupal integument of S. exigua through race PCR Strategy. The deduced amino acid sequences were found to contain the RR-2 consensus region of other insect cuticular proteins and construct phylogenetic trees for each protein. Using quantitative RT-PCR, the developmental expression of the three genes through several larval and the early pupal stages was studied. All three genes contribute to the endocuticle although CPG316 may have a different role from the other two genes. All three newly isolated genes were analyzed and their functions were determi

SUBMITTER: Jan S 

PROVIDER: S-EPMC5700046 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature