Reassessment of chitosanase substrate specificities and classification.

ABSTRACT: Chitosanases can be used to produce partially acetylated chitosan oligosaccharides (paCOS) for different applications, provided they are thoroughly characterized. However, recent studies indicate that the established classification system for chitosanases is too simplistic. Here, we apply a highly sensitive method for quantitatively sequencing paCOS to reassess the substrate specificities of the best-characterized class I-III chitosanases. The enzymes' abilities to cleave bonds at GlcNAc residues positioned at subsite (-1) or (+1), on which the classification system is based, vary especially when the substrates have different fractions of acetylation (F _A ). Conflicts with the recent classification are observed at higher F _A , which were not investigated in prior spec

SUBMITTER: Weikert T 

PROVIDER: S-EPMC5700058 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature