Two Pools of Vesicles Associated with Synaptic Ribbons Are Molecularly Prepared for Release.

ABSTRACT: Neurons that form ribbon-style synapses are specialized for continuous exocytosis. To this end, their synaptic terminals contain numerous synaptic vesicles, some of which are ribbon associated, that have difference susceptibilities for undergoing Ca²⁺-dependent exocytosis. In this study, we probed the relationship between previously defined vesicle populations and determined their fusion competency with respect to SNARE complex formation. We found that both the rapidly releasing vesicle pool and the releasable vesicle pool of the retinal bipolar cell are situated at the ribbon-style active zones, where they functionally interact. A peptide inhibitor of SNARE complex formation failed to block exocytosis from either pool, suggesting that these two vesicle pools have formed the SNA

SUBMITTER: Datta P 

PROVIDER: S-EPMC5700246 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature