Single Nucleotide Polymorphism Genotyping in Single-Molecule Electronic Circuits.

ABSTRACT: Establishing low-cost, high-throughput, simple, and accurate single nucleotide polymorphism (SNP) genotyping techniques is beneficial for understanding the intrinsic relationship between individual genetic variations and their biological functions on a genomic scale. Here, a straightforward and reliable single-molecule approach is demonstrated for precise SNP authentication by directly measuring the fluctuations in electrical signals in an electronic circuit, which is fabricated from a high-gain field-effect silicon nanowire decorated with a single hairpin DNA, in the presence of different target DNAs. By simply comparing the proportion difference of a probe-target duplex structure throughout the process, this study implements allele-specific and accurate SNP detection. These results are s

SUBMITTER: He G 

PROVIDER: S-EPMC5700462 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature