Methylomic and phenotypic analysis of the ModH5 phasevarion of Helicobacter pylori.

ABSTRACT: The Helicobacter pylori phase variable gene modH, typified by gene HP1522 in strain 26695, encodes a N⁶-adenosine type III DNA methyltransferase. Our previous studies identified multiple strain-specific modH variants (modH1 - modH19) and showed that phase variation of modH5 in H. pylori P12 influenced expression of motility-associated genes and outer membrane protein gene hopG. However, the ModH5 DNA recognition motif and the mechanism by which ModH5 controls gene expression were unknown. Here, using comparative single molecule real-time sequencing, we identify the DNA site methylated by ModH5 as 5'-G^m6ACC-3'. This motif is vastly underrepresented in H. pylori genomes, but overrepresented in a number of virulence genes, including motility-associated genes, and outer m

SUBMITTER: Srikhanta YN 

PROVIDER: S-EPMC5700931 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature