Genome-wide analysis of E. coli cell-gene interactions.

ABSTRACT:

Background

The pursuit of standardization and reliability in synthetic biology has achieved, in recent years, a number of advances in the design of more predictable genetic parts for biological circuits. However, even with the development of high-throughput screening methods and whole-cell models, it is still not possible to predict reliably how a synthetic genetic construct interacts with all cellular endogenous systems. This study presents a genome-wide analysis of how the expression of synthetic genes is affected by systematic perturbations of cellular functions. We found that most perturbations modulate expression indirectly through an effect on cell size, putting forward the existence of a generic Size-Expression interaction in the model prokaryote Escherichia coli.

Results

SUBMITTER: Cardinale S 

PROVIDER: S-EPMC5701387 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature