Superoxide Scavenging and Antiglycation Activity of Rhinacanthins-rich Extract Obtained from the Leaves of Rhinacanthus nasutus.

ABSTRACT:

Background

Oxidative stress and nonenzymatic protein glycation lead to serious diabetic complications that increase the risk of mortality. Rhinacanthus nasutus leaf crude extracts are previously reported for their antidiabetic, antiglycation, and antioxidant potential.

Objective

The present study was performed to prepare a standardized rhinacanthins-rich extract (RRE) and evaluate its superoxide scavenging and antiglycation effects as compared to its marker compounds, namely, rhinacanthin-C (RC), rhinacanthin-D (RD), and rhinacanthin-N (RN).

Materials and methods

RRE was obtained by microwave-assisted green extraction along with a simple step of fractionation using Amberlite^® column. RC, RD, and RN were isolated from the RRE using silica gel column c

SUBMITTER: Shah MA 

PROVIDER: S-EPMC5701406 | biostudies-literature | 2017 Oct-Dec

REPOSITORIES: biostudies-literature