Tracking maize pollen development by the Leaf Collar Method.

ABSTRACT:

Key message

An easy and highly reproducible nondestructive method named the Leaf Collar Method is described to identify and characterize the different stages of pollen development in maize. In plants, many cellular events such as meiosis, asymmetric cell division, cell cycle regulation, cell fate determination, nucleus movement, vacuole formation, chromatin condensation and epigenetic modifications take place during pollen development. In maize, pollen development occurs in tassels that are confined within the internal stalk of the plant. Hence, identification of the different pollen developmental stages as a tool to investigate above biological processes is impossible without dissecting the entire plant. Therefore, an efficient and reproducible method is necessary to isolate homog

SUBMITTER: Begcy K 

PROVIDER: S-EPMC5701949 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature