Distinct intracellular sAC-cAMP domains regulate ER Ca²⁺ signaling and OXPHOS function.

ABSTRACT: cAMP regulates a wide variety of physiological functions in mammals. This single second messenger can regulate multiple, seemingly disparate functions within independently regulated cell compartments. We have previously identified one such compartment inside the matrix of the mitochondria, where soluble adenylyl cyclase (sAC) regulates oxidative phosphorylation (OXPHOS). We now show that sAC knockout fibroblasts have a defect in OXPHOS activity and attempt to compensate for this defect by increasing OXPHOS proteins. Importantly, sAC knockout cells also exhibit decreased probability of endoplasmic reticulum (ER) Ca²⁺ release associated with diminished phosphorylation of the inositol 3-phosphate receptor. Restoring sAC expression exclusively in the mitochondrial matrix rescues OXP

SUBMITTER: Valsecchi F 

PROVIDER: S-EPMC5702058 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature