Automated growth rate determination in high-throughput microbioreactor systems.

ABSTRACT:

Objective

The calculation of growth rates provides basic metric for biological fitness and is standard task when using microbioreactors (MBRs) in microbial phenotyping. MBRs easily produce huge data at high frequency from parallelized high-throughput cultivations with online monitoring of biomass formation at high temporal resolution. Resulting high-density data need to be processed efficiently to accelerate experimental throughput.

Results

A MATLAB code is presented that detects the exponential growth phase from multiple microbial cultivations in an iterative procedure based on several criteria, according to the model of exponential growth. These were obtained with Corynebacterium glutamicum showing single exponential growth phase and Escherichia coli exhibiting diauxic gro

SUBMITTER: Hemmerich J 

PROVIDER: S-EPMC5702135 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature