Genome-wide segregation of single nucleotide and structural variants into single cancer cells.

ABSTRACT:

Background

Single-cell genome sequencing provides high-resolution details of the clonal genomic modifications that occur during cancer initiation, progression, and ongoing evolution as patients undergo treatment. One limitation of current single-cell sequencing strategies is a suboptimal capacity to detect all classes of single-nucleotide and structural variants in the same cells.

Results

Here we present a new approach for determining comprehensive variant profiles of single cells using a microfluidic amplicon-based strategy to detect structural variant breakpoint sequences instead of using relative read depth to infer copy number changes. This method can reconstruct the clonal architecture and mutational history of a malignancy using all classes and sizes of somatic variant

SUBMITTER: Easton J 

PROVIDER: S-EPMC5702214 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature