Triple-Stage Mass Spectrometry Unravels the Heterogeneity of an Endogenous Protein Complex.

ABSTRACT: Protein complexes often represent an ensemble of different assemblies with distinct functions and regulation. This increased complexity is enabled by the variety of protein diversification mechanisms that exist at every step of the protein biosynthesis pathway, such as alternative splicing and post transcriptional and translational modifications. The resulting variation in subunits can generate compositionally distinct protein assemblies. These different forms of a single protein complex may comprise functional variances that enable response and adaptation to varying cellular conditions. Despite the biological importance of this layer of complexity, relatively little is known about the compositional heterogeneity of protein complexes, mostly due to technical barriers of studying such close

SUBMITTER: Ben-Nissan G 

PROVIDER: S-EPMC5702261 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature