Novel peptide probes to assess the tensional state of fibronectin fibers in cancer.

ABSTRACT: Transformations of extracellular matrix (ECM) accompany pathological tissue changes, yet how cell-ECM crosstalk drives these processes remains unknown as adequate tools to probe forces or mechanical strains in tissues are lacking. Here, we introduce a new nanoprobe to assess the mechanical strain of fibronectin (Fn) fibers in tissue, based on the bacterial Fn-binding peptide FnBPA5. FnBPA5 exhibits nM binding affinity to relaxed, but not stretched Fn fibers and is shown to exhibit strain-sensitive ECM binding in cell culture in a comparison with an established Fn-FRET probe. Staining of tumor tissue cryosections shows large regions of relaxed Fn fibers and injection of radiolabeled ¹¹¹In-FnBPA5 in a prostate cancer mouse model reveals specific accumulation of ¹¹¹In-Fn

SUBMITTER: Arnoldini S 

PROVIDER: S-EPMC5702617 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature