Second-generation method for analysis of chromatin binding with formaldehyde-cross-linking kinetics.

ABSTRACT: Formaldehyde-cross-linking underpins many of the most commonly used experimental approaches in the chromatin field, especially in capturing site-specific protein-DNA interactions. Extending such assays to assess the stability and binding kinetics of protein-DNA interactions is more challenging, requiring absolute measurements with a relatively high degree of physical precision. We previously described an experimental framework called the cross-linking kinetics (CLK) assay, which uses time-dependent formaldehyde-cross-linking data to extract kinetic parameters of chromatin binding. Many aspects of formaldehyde behavior in cells are unknown or undocumented, however, and could potentially affect CLK data analyses. Here, we report biochemical results that better define the properties of formal

SUBMITTER: Zaidi H 

PROVIDER: S-EPMC5702673 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature