Various calibration procedures result in optimal standardization of routinely used 25(OH)D ID-LC-MS/MS methods.

ABSTRACT:

Background

The variety of LC-MS/MS methods measuring total 25(OH)D used today is vast and the comparability among these methods is still not well assessed.

Methods

Here, we performed a comparison in samples of healthy donors between the currently routinely used 25(OH)D LC-MS/MS methods in the Netherlands and the Ghent University reference measurement procedure to address this issue (n=40). Additionally, an interlaboratory comparison in patient serum samples assessed agreement between the Dutch diagnostic methods (n=37).

Results

The overall correlation of the routine methods for 25(OH)D₃ with the reference measurement procedures and with the mean of all diagnostic methods was excellent (r>0.993 and r>0.989, respectively). Three out of five methods aligned pe

SUBMITTER: Dirks NF 

PROVIDER: S-EPMC5703036 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature