Myoanatomy of the velvet worm leg revealed by laboratory-based nanofocus X-ray source tomography.

ABSTRACT: X-ray computed tomography (CT) is a powerful noninvasive technique for investigating the inner structure of objects and organisms. However, the resolution of laboratory CT systems is typically limited to the micrometer range. In this paper, we present a table-top nanoCT system in conjunction with standard processing tools that is able to routinely reach resolutions down to 100 nm without using X-ray optics. We demonstrate its potential for biological investigations by imaging a walking appendage of Euperipatoides rowelli, a representative of Onychophora-an invertebrate group pivotal for understanding animal evolution. Comparative analyses proved that the nanoCT can depict the external morphology of the limb with an image quality similar to scanning electron microscopy, while simulta

SUBMITTER: Muller M 

PROVIDER: S-EPMC5703297 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature