Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.

ABSTRACT:

Aim

Interleukin-27 (IL-27) is involved in different inflammatory diseases; however, its role in atherosclerosis is unclear. In this study we investigated the expression of IL-27 and its receptor in patients with carotid atherosclerosis and if IL-27 could modulate the inflammatory effects of the NLRP3 inflammasome in vitro.

Methods

Plasma IL-27 was measured by enzyme immunoassay in patients with carotid stenosis (n = 140) and in healthy controls (n = 19). Expression of IL-27 and IL-27R was analyzed by quantitative PCR and immunohistochemistry in plaques from patients and in non-atherosclerotic vessels. THP-1 monocytes, primary monocytes and peripheral blood mononuclear cells (PBMCs) were used to study effects of IL-27 in vitro.

Results

Our main findings were: (i) Plasm

SUBMITTER: Gregersen I 

PROVIDER: S-EPMC5703457 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature