Investigating dye performance and crosstalk in fluorescence enabled bioimaging using a model system.

ABSTRACT: Detailed imaging of biological structures, often smaller than the diffraction limit, is possible in fluorescence microscopy due to the molecular size and photophysical properties of fluorescent probes. Advances in hardware and multiple providers of high-end bioimaging makes comparing images between studies and between research groups very difficult. Therefore, we suggest a model system to benchmark instrumentation, methods and staining procedures. The system we introduce is based on doped zeolites in stained polyvinyl alcohol (PVA) films: a highly accessible model system which has the properties needed to act as a benchmark in bioimaging experiments. Rather than comparing molecular probes and imaging methods in complicated biological systems, we demonstrate that the model system can emulat

SUBMITTER: Arppe R 

PROVIDER: S-EPMC5703511 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature